Flavonoids Induce Migration Arrest and Apoptosis in Detroit 562 Oropharynx Squamous Cell Carcinoma Cells

Despite advances in the treatment of head and neck squamous cell carcinoma (HNSCC), morbidity remains at a high level due to resistance SCC cells chemotherapeutics. This study aimed determine compare magnitude flavonoids’ effectiveness activating apoptosis migration arrest HNSCC vitro. Methods: Head Detroit 562 line were exposed range concentrations (5–100 μM) quercetin (Que), hesperidin (Hes) rutin (Rut) for 24 48 h. The viability rate investigated using cytotoxicity inhibition assays. Muse Cell Analyzer flow cytometry was utilized quantitatively assess Que, Hes Rut. morphology evaluated via hematoxylin-eosin staining. Results: diminishment 562-line treated with Rut h revealed significant dose-dependent trend, relatively equal three substances, whereas most noticeable cytotoxic effect observed Hes. Exposure exhibited increased proportion apoptotic cells, either necrosis or late stage. motility halted 100 µM dose Que. comparative results elucidated that Hesperidin Quercetin achieved more potent reduction Conclusions: Hesperidin, are capable inducing various extents, resulting proapoptotic attenuating effects different magnitudes.